Iv been removing cannabinoids from my fractions with d- Limonene as my non polar solvent, I followed beaker’s guide but I’m stubborn, so I go through a lot, and I mean a lot of d-limonene because some cannabinoids are more polar then others.
Any solvent that I could use in conjunction with my D-limonene to increase polarity?
No i am sure just acetone is fine(no water because it is hard to remove), diethyl ether is best because it is easier to remove (low BP). So wash your fractions with acetone 3 times then pour some in and take a tlc of the solution in the fraction to see if there are any compounds left in the glass fraction.
My understanding is he was just asking about cleaning out his fractions not actually running the column. Yes do not run columns in acetone unless you are using reverse phase C18 silica gel
I remind the forum, if you have regular phase silica gel (polar 60-300 micrometer mesh) the general way to elute very polar substrates is with 10% MeOH in methylene chloride (do not exceed 10% MeOH or you will dissolve some of your silica gel or some other shit could happen according to march’s advanced organic chemistry 5th-7th ed).
Or use my personal favorite recipe for the elution of polar compounds: Polar express: 3:1:2% ethyl acetate:ethanol:acetic acid