I see, or think I do…washing the transformed cell line prior to extraction.? I did a bit of work on serotonin biosynthesis in living tissue, in vitro preparations.
Do the centrifugal units have a name? Did you just invent that…trying to read the diagram symbolically, does it even mean centrifugal? Or are they just percolation LLE units?
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when we are doing the chemical conversions, some of the material doesnt have the reaction as expected and is un-spent. so we need to get that material out of there since its not the active we are looking for. Its water soluble, therefore we do staged water washes with precise dosing per amounts of water.
just centrifugal LLE. we got these from china, each unit was about $3200 delivered, lot of money on that row of tables.
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Do you mean base to salts? Or are you actually making adducts of the 5OH-DMT? I am not that interested in details of what you are doing., trying to get a read on the utility of serial- centrifugal based separation. That is to say in contrast to CPC which I am sure generates far more plates. If the answer lies in some “proprietary method” file you don’t want to disclose..that’s ok…..I found a good review, …thanks.
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5-meo-dmt
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its not rocket science. water flows in, solvent is dosed in with metered pumps, it spins around with centrifugal force, the water touches the solvent and pulls material out of solvent, and then its separated with centrifugal force and separated back into solvent and water.
you can do like we do and run 5 stages so its doing the process 5 times per “run” and then its going into barrels separated, then we do another process with a different type of water and going back and forth to achieve multiple types of washes and multiple washes per type. doing it in flow is very very fast instead of doing it in batches
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The water removing 5-meo-nmt, 5-meo-tryptamine, formaldehyde I presume?
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bingo. mostly un-converted 5-meo-tryptamine
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Does this process work for removing the nmt and tryptamine? I was unaware of any aqueous solubility difference. I was recommended to form the NMT carboxylate as a way to separate.
Hmm I didn’t know that! Interesting. Of course NMTs can be largely mitigated as long as you are performing the Eschweiler-Clarke right.
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if done right you have zero nmt
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