I see, or think I do…washing the transformed cell line prior to extraction.? I did a bit of work on serotonin biosynthesis in living tissue, in vitro preparations.
Do the centrifugal units have a name? Did you just invent that…trying to read the diagram symbolically, does it even mean centrifugal? Or are they just percolation LLE units?
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when we are doing the chemical conversions, some of the material doesnt have the reaction as expected and is un-spent. so we need to get that material out of there since its not the active we are looking for. Its water soluble, therefore we do staged water washes with precise dosing per amounts of water.
just centrifugal LLE. we got these from china, each unit was about $3200 delivered, lot of money on that row of tables.
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Do you mean base to salts? Or are you actually making adducts of the 5OH-DMT? I am not that interested in details of what you are doing., trying to get a read on the utility of serial- centrifugal based separation. That is to say in contrast to CPC which I am sure generates far more plates. If the answer lies in some “proprietary method” file you don’t want to disclose..that’s ok…..I found a good review, …thanks.
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5-meo-dmt
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its not rocket science. water flows in, solvent is dosed in with metered pumps, it spins around with centrifugal force, the water touches the solvent and pulls material out of solvent, and then its separated with centrifugal force and separated back into solvent and water.
you can do like we do and run 5 stages so its doing the process 5 times per “run” and then its going into barrels separated, then we do another process with a different type of water and going back and forth to achieve multiple types of washes and multiple washes per type. doing it in flow is very very fast instead of doing it in batches
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The water removing 5-meo-nmt, 5-meo-tryptamine, formaldehyde I presume?
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bingo. mostly un-converted 5-meo-tryptamine
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Does this process work for removing the nmt and tryptamine? I was unaware of any aqueous solubility difference. I was recommended to form the NMT carboxylate as a way to separate.
Hmm I didn’t know that! Interesting. Of course NMTs can be largely mitigated as long as you are performing the Eschweiler-Clarke right.
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if done right you have zero nmt
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Yes, understood. 5-meo-dmt, an interesting 5HT2a-R full agaonist. I am interested in whether you can hit that up (at a correct temp) in a vape pipe or does it lose the .methoxy on vaporization ? Any first hand data on that subject.
Because some of the 2a receptor full agonists , are balanced with respect beta arrestin activation and resulting internalization of the receptor….I am wondering is a quick hit of a vape pipe loaded with 5-meo-dmt, might be beneficial for those who suffer from (Non-Suicidal Self-Injury Disorder (NSSID)) , like cutting It might be that taking a hit off the pipe relieves the intense anxiety situation?
Any anecdotal comments in these regards.?
@oscar ?
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Absolutely! Same with n,n-dmt. We have some members of the church that will take the smallest puffs off the vape pen before meetings and other stressful situations and they claim that it is a miracle drug for them.
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You are on to something, Either Vaping or Nebulizing in Saline may help thousands for instant relief.
@Ganjineer710 may have great ideas in regards to the latter.
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Would you be willing to share the vendor for these?
Its no surprise that we are going through close to 30,000 dmt pens a month at the church. The conversations we have with members are wild….
Here is something that our amazing government has no fucking clue about, they ban these substances claiming no real medical benefits but we know something they dont know…. DMT, can be fun, life changing, transformative, and more…but…what it and these other psychedelics do is affect parasites that live inside us.
people dont consider parasites and what they do to the nervous system and how they hijack what we think of as “reality” by controlling mood, emotions, as well as what we see…if you can hijack the nervous system you can do all sorts of nefarious things.
We have started a protocol to help people SEE the contrast when parasites are disconnected, to see their real self on/off parasites and then they are able to jump on the procedure to remove the parasites fully and turn on that “real version of themselves full time”
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We sell these units. lead time is 8 weeks. A lot of work went into fine tuning and making changes to the systems to work like we wanted them to.
nebulalizer™️
I’m a little more interested in nebulization of 4-HO-DMT but am having trouble with shelf-life. n,n-dmt and 5-meo seem to work fine in the traditional heated atomizer.
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Why 4-OH?
I should caution against just using sort of randomly hydroxylated biogenic amines in configuration not normally found in the nervous system.
For instance, 6-OH Dopamine. Can produce a one dose , permanent destruction of dopamine cells in brain stem…and an instant Parkinson’s like state.
3, 4, 0r 5 ok, 6 toxic.
I just have concerns…where is 4OH found natural ? Do you know its toxicity.
that aside …How are you preparing your nebulized product.
The indol amines like Catecholamines can undergo a number of reactions , with other compounds and them selves…in what form do you get these chemical. Well, dmt has always been smoked in western cultures. (Amazon.snuffs)..
??? In a discussion with Albert Hoffman, he mentioned subcutaneous injections.
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we have done n,n-dmt patches as well as pharmahuasca with pharma grade reversible maoi. I would stay away from all these odd synthetic things…. 5-meo natural, n,n-dmt natural…. you really dont need much more
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